Single-Step Scalable-Throughput Molecular Screening for Huntington Disease

Single-step scalable-throughput molecular screening for Huntington disease.

BACKGROUND Huntington disease (HD) is a fatal autosomal dominant neurodegenerative disorder caused by an unstable expansion of the CAG trinucleotide repeat in exon 1 of the HTT (huntingtin) gene and typically has an adult onset. Molecular diagnosis and screening for HD currently involve separate amplification and detection steps. METHODS We evaluated a novel, rapid microplate-based screening ...

Huntington disease: prenatal screening for late onset disease.

Screening some Iranian Muskmelon Landraces for Resistance Against Fusarium Wilt Disease using Molecular Markers

Fusarium wilt is one of the most destructive diseases of muskmelon (Cucumis melo L.), which is an economically important disease worldwide causes yield losses in muskmelon growing areas. One of the most effective controlling measures to prevent Fusarium wilt is through host resistance by using resistance genes. We used developed molecular markers for Fom-2 gene, which confers ...

Screening for a Single Disease

We should begin by making a distinction between the terms "screening" and "diagnosis." The purpose of screening is to select from an apparently healthy population those who display sufficient probability of an illness to warrant referral for diagnosis. As defined by the National Conference on Chronic Diseases [47] (cited in [16]): "Screening is the presumptive identification of unrecognized dis...

Droplet microfluidic technology for single-cell high-throughput screening.

We present a droplet-based microfluidic technology that enables high-throughput screening of single mammalian cells. This integrated platform allows for the encapsulation of single cells and reagents in independent aqueous microdroplets (1 pL to 10 nL volumes) dispersed in an immiscible carrier oil and enables the digital manipulation of these reactors at a very high-throughput. Here, we valida...